Denatured protein should not be confused with Fibrin, a fine precipitate sometimes seen in thawed serum. Effect of recombinant canine interleukin-6 and interleukin-8 on tissue factor procoagulant activity in canine peripheral blood mononuclear cells and purified canine monocytes.

Objective: This site needs JavaScript to work properly. Heated for 30 minutes at 56°C with mixing to inactivate complement proteins that are part of the immune response. After 30 minutes turn off the oscillating shaker and remove the bottles from the water bath. Optimization of conditions for in vitro production of radical oxygen species and expression of tissue factor by canine mononuclear cells and granulocytes for use in high-throughput assays.

eCollection 2013. Moon SH, Kim SM, Park SJ, Kim H, Bae D, Choi YS, Chung HM. Removal of complement activity from the serum is not required for most cell cultures, but may be necessary for cultures that are sensitive to the complement activity.

Epub 2015 Jan 8. The objective of heat inactivation is to destroy complement activity in the serum without affecting the growth-promoting characteristics of the product.

Swirl bottle thoroughly every 10 minutes or if applicable turn on the oscillating shaker unit. Equalfetal™, Fetal Plus®, Fetal Reserve, Fetal Select™™, and Fetal Choice are trademarks, registered trademarks, or proprietary to Atlas Biologicals.  | 

Procedures: It should not be assumed that heat-inactivation of fetal bovine serum is needed or even appropriate.

Any use of them is prohibited. Why Use Heat-inactivated Fetal Bovine Serum? Keep in mind that heat inactivation requires the serum to be at 56°C for 30 minutes. Order serum with heat inactivation already completed.Simply request heat inactivation when ordering.
Controls bottles are volumed to the same level as the product being heat inactivated and fitted with a thermometer suitable for monitoring 56°C. Addition of HI-FBS to media did not stimulate procoagulant activity of equine or canine cells, and the sensitivity of the equine cells to LPS was significantly increased by HI-FBS. Figueiredo MD, Moore JN, Vandenplas ML, Sun WC, Murray TF.


Atlas Biologicals is NOT affiliated with, split off, a spin-off, a subsidiary, associated with, a sister company, partner, or related to any other company or corporation. Find NCBI SARS-CoV-2 literature, sequence, and clinical content: https://www.ncbi.nlm.nih.gov/sars-cov-2/. Vet Immunol Immunopathol.

Am J Vet Res.

Epub 2006 Jun 5. NLM Specifications The temperature of the control must be 56± 2°C for 30 ± 2 minutes during the heat inactivation process.

Please enable it to take advantage of the complete set of features! A 1 stage recalcification assay was used to determine procoagulant activity.

This is very different than putting the serum in a 56°C water bath for 30 minutes, which is not heat inactivation by definition. Increasing concentrations of HIFBS significantly increased LPS- and zymosan-induced procoagulant activity of canine cells. FBS is a cocktail of proteins, vitamins, carbohydrates, lipids, hormones, growth factors, minerals and trace elements and is used as an universal growth supplement effective for most types of human and animal (including insect) cells.


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